On an interspecific level, including 11 ascidian species screened in a pilot study, fouling-deterring activities were correlated with epibacterial abundances while antimicrobial activity was not.
lobatus colonies exhibited varying, but generally low, levels of antiraicrobial activity against, on average, one-half of the 36 strains of marine bacteria tested. In addition to reducing bacterial settlement, extracts of C. planum induce settlement relative to the control. lobatus deter bacterial settlement while extracts of the heavily colonized P. The results of the settlement bioassay clearly demonstrate that extracts of the little-colonized C. Bacterial settlement was measured using a new bioassay described in this paper. Organic extracts of both ascidian species were tested for antimicrobial activities and effects on bacterial settlement. planum were 140 times greater than those on C lobatus as measured by plate-counting methods. Two co-occurring ascidians of the Pacific subtidal, Polyclinum planum and Cystodytes lobatus, were found to exhibit remarkably different numbers of surface bacteria. Molecular characterization of the 16S rRNA gene by PCR, Bacillus subtilis strain BAB-684 identification for isolates one is the number of nucleotide pairs reached 899 bp and the degree of similarity in GenBank reached 89% homologous, while the second is a Bacillus flexus strain PHCDB20 isolates the number reached 950 bp nucleotide pairs with the degree of similarity in GenBank reached 99% homologous. Characteristics of Isolates 1 spherical shape, colonize and creamy, while isolates 2 jagged shape, oval and white colonies.
Isolation and purification mikro symbionts of sponge obtained 2 (two) isolates.
The results of histo-morphological analysis concluded sponge samples with species of Callyspongia sp. The research method that morphological identification, isolation-purification and molecular characterization of the 16S rRNA gene with Chain Reaction Polymerization method. Objective analysis of histo-morphological research, isolation-purification, molecular characterization of micro-symbiont genes in order to search symbiont bacteria that can live in extreme environments contaminated hydrocarbon waste. Molecular characterization studies have been conducted 16S rRNA gene micro symbiont of sponge origin Melawai Beach, Balikpapan in East Kalimantan.
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